AGO2 Knockout cell line (THP-1)

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AGO2 Knockout cell line (THP-1) Order

AGO2 Knockout cell line (THP-1)
Catalog#
YKO-HT1324
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AGO2 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AGO2 Knockout cell line (THP-1)
Species
Human
Gene Name
AGO2
Gene ID
27161
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript AGO2-201)
Validation: PCR+Sanger Sequencing
AGO2 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AGO2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AGO2 gene knockout AGO2 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AGO2 Knockout cell line (THP-1)
AGO2 Knockout cell line (THP-1)
Catalog#
YKO-HT1324
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)
      inquiry   More details

    Organism:
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1477
    YKO-H1477(HeLa)
  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-HS13754
    YKO-HS13754(Hela)
  • AGO2 knockout cell line (HCT 116)

    AGO2 knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1254
    YKO-H1254(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)

    Organism:
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1477
      inquiry   More details
  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-HS13754
      inquiry   More details
  • AGO2 knockout cell line (HCT 116)

    AGO2 knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1254
      inquiry   More details

AGO2 Knockout cell line (THP-1) Order

AGO2 Knockout cell line (THP-1)
Catalog#
YKO-HT1324
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AGO2 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AGO2 Knockout cell line (THP-1)
Species
Human
Gene Name
AGO2
Gene ID
27161
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript AGO2-201)
Validation: PCR+Sanger Sequencing
AGO2 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AGO2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AGO2 gene knockout AGO2 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AGO2 Knockout cell line (THP-1)
AGO2 Knockout cell line (THP-1)
Catalog#
YKO-HT1324
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)
      inquiry   More details

    Organism:
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1477
    YKO-H1477(HeLa)
  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-HS13754
    YKO-HS13754(Hela)
  • AGO2 knockout cell line (HCT 116)

    AGO2 knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1254
    YKO-H1254(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)

    Organism:
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1477
      inquiry   More details
  • AGO2 Knockout cell line (HeLa)

    AGO2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-HS13754
      inquiry   More details
  • AGO2 knockout cell line (HCT 116)

    AGO2 knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AGO2
    Gene ID: 27161
    Size: 1*10^6
    Catalog#: YKO-H1254
      inquiry   More details

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